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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
25/10/2012 |
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Data da última atualização: |
31/10/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CALEGARIO, R. F.; LABATE, M. T. V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W. |
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Afiliação: |
RENATA FAIER CALEGARIO, ESALQ; MÔNICA TERESA VENEZIANO LABATE, ESALQ; LUÍS ANTÔNIO PERONI, UNICAMP; DAGMAR RUTH STACH-MACHADO, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; CARLOS ALBERTO LABATE, ESALQ; MARCOS ANTÔNIO MACHADO, Centro APTA Citros Sylvio Moreira; ELLIOT WATANABE KITAJIMA, ESALQ. |
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Título: |
In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Tropical Plant Pathology, n. 2, v. 37, p. 255-265, March-April, 2012. |
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ISSN: |
1982-5676 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter. Key words: Brevipalpus phoenicis, Cilevirus, Citrus sinensis, polyclonal antibody, serology. |
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Palavras-Chave: |
Polyclonal antibody. |
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Thesagro: |
Brevipalpus Phoenicis; Citrus Sinensis. |
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Thesaurus NAL: |
Cilevirus; Citrus; Citrus leprosis virus C; serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68741/1/in-vitro-expression.pdf
|
|
Marc: |
LEADER 02278naa a2200301 a 4500
001 1937928
005 2012-10-31
008 2012 bl uuuu u00u1 u #d
022 $a1982-5676
100 1 $aCALEGARIO, R. F.
245 $aIn vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C).
260 $c2012
520 $aCitrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter. Key words: Brevipalpus phoenicis, Cilevirus, Citrus sinensis, polyclonal antibody, serology.
650 $aCilevirus
650 $aCitrus
650 $aCitrus leprosis virus C
650 $aserology
650 $aBrevipalpus Phoenicis
650 $aCitrus Sinensis
653 $aPolyclonal antibody
700 1 $aLABATE, M. T. V.
700 1 $aPERONI, LUÍS A.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aASTUA, J. de F.
700 1 $aLABATE, C. A.
700 1 $aMACHADO, M. A.
700 1 $aKITAJIMA, E. W.
773 $tTropical Plant Pathology$gn. 2
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Embrapa Mandioca e Fruticultura (CNPMF) |
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